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  1. Home
  2. Cleaning Services
  3. Bio-One
Bio-One logo
Cleaning Services · Crime/Trauma Scene Cleaning

Bio-One- As a Franchise Owner, you'll support and care for others during life's most difficult moments. Serve your community by providing a wide range of cleanup services that also create multiple revenue streams for your business. You don’t need any previous experience to own a Bio-One franchise. Just bring your heart for service and we’ll provide you with all the tools and training you need to run your business. - Recession-Resilient: Our services are consistently in demand, regardless of economic factors. - Non-Seasonal: Bio-One operates 24/7/365, ensuring continuous business regardless of the season. - Limited Competition: In a growing industry with few competitors, your business stands out locally. - Limited Staff Needed: Few technicians are required for each job, streamlining operations. - Category-Leading Brand: Bio-One has empowered owner success since 2010 as the industry's largest and first-to-franchise in this niche. - High Tickets & Strong Margins: We specialize in high-value insurance-based jobs with attractive margins. - Fast Start-Up: We offer comprehensive training and support, ensuring a swift and efficient opening and ongoing operation. - Low Start-Up Costs: Our business model features low initial investment and scalability, with no need for expensive equipment. - Manageable Labor: Start with a few highly trained technicians to kickstart your business. - Five Star Support: Benefit from specialized training, expertise, partnerships, and ongoing support to maximize your growth potential. - Onboarding Support - Website, SEO, PPC, Google Business Profile /OnlineListings, Facebook/Social - Local Marketing Tools and Resources - National Branding / Brand Guidelines - National Sponsorships / Partnerships and Marketing Events

Home-BasedSBA Approved
Total Investment

$135K - $221K

Franchise Fee

$60K

Royalty

7.5%

Ad Fund

—

Term

—

Open Units

—

Overview

About Bio-One

- As a Franchise Owner, you'll support and care for others during life's most difficult moments. Serve your community by providing a wide range of cleanup services that also create multiple revenue streams for your business. You don’t need any previous experience to own a Bio-One franchise. Just bring your heart for service and we’ll provide you with all the tools and training you need to run your business. - Recession-Resilient: Our services are consistently in demand, regardless of economic factors. - Non-Seasonal: Bio-One operates 24/7/365, ensuring continuous business regardless of the season. - Limited Competition: In a growing industry with few competitors, your business stands out locally. - Limited Staff Needed: Few technicians are required for each job, streamlining operations. - Category-Leading Brand: Bio-One has empowered owner success since 2010 as the industry's largest and first-to-franchise in this niche. - High Tickets & Strong Margins: We specialize in high-value insurance-based jobs with attractive margins. - Fast Start-Up: We offer comprehensive training and support, ensuring a swift and efficient opening and ongoing operation. - Low Start-Up Costs: Our business model features low initial investment and scalability, with no need for expensive equipment. - Manageable Labor: Start with a few highly trained technicians to kickstart your business. - Five Star Support: Benefit from specialized training, expertise, partnerships, and ongoing support to maximize your growth potential. - Onboarding Support - Website, SEO, PPC, Google Business Profile /OnlineListings, Facebook/Social - Local Marketing Tools and Resources - National Branding / Brand Guidelines - National Sponsorships / Partnerships and Marketing Events

Bio-One Inc. is the category-leader and first in biohazard and decontamination cleanup services growing quickly to 100+ franchise owners operating 130+ locations. Helping people and communities in their greatest times of need, Bio-one answers the call with discretion, care, and compassion.. Bio-One believes in a simple and clear mission: Help First, Business Second. A close-knit brand of community heroes devoted to cleaning up what others won’t (or can’t) including biohazard cleanup, crime scene cleanup, hoarding cleanup, mold remediation, and more. Bio-One franchisees are trained to handle these situations with compassion and professionalism, providing a valuable service to their communities. Acquired by Five Star Franchising in 2021, Bio-One is well-positioned and supported for rapid expansion and growth across North America. With low start-up costs and high profit margins, this recession-resilient industry provides an opportunity for strong leaders who are passionate about making a difference in their community.

Who it fits

Ideal candidate

Passion to Help PeopleNo Industry Experience RequiredCommunity CentricPassion for Health/WellnessBusiness / Operations / Sales LeadershipPersonable, Strong Communicator, Leader and Relationship BuilderTenacity for Brand Stewardship and Customer ExperienceDesire to Solve Challenges for and Help Clients in their Communities
The fine print

FDD facts

Initial franchise fee$60K
Royalty7.5%
Total investment$135K - $221K
Liquid capital required$60K
Net worth requirement$250K
Year founded2006
Year began franchising2010
Business modelBoth
Owner-operator required (Year 1)Yes
Reported figures from public FDD disclosures and franchisor websites. Verify with the official FDD before signing.
Footprint

Territory overview

AvailableGeorgia, New York, Idaho, Pennsylvania, Massachusetts, Minnesota, Tennessee, Washington, Illinois
UnavailableAlabama, Arizona, Colorado, Missouri, Nevada, Ohio, Wyoming, Florida, North Carolina, Texas, Utah, California, South Carolina, New Jersey
LegendAvailableLimitedUnavailable
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Quick facts
Founded
2006
Franchising since
2010
Brand family
Five Star Franchising
Model
Both
Liquid capital
$60K min
Net worth
$250K min
Owner-op required?
Yes
Side-by-side

See how Bio-One stacks against another brand on every Item 19 number.

Data verified 2026
Sourced from public FDD filings & franchisor disclosures.
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